4YJ6
The Crystal Structure of a Bacterial Aryl Acylamidase Belonging to the Amidase signature (AS) enzymes family
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 75.760, 75.760, 214.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.700 - 1.700 |
R-factor | 0.1553 |
Rwork | 0.155 |
R-free | 0.16790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f2a |
RMSD bond length | 0.014 |
RMSD bond angle | 1.454 |
Data reduction software | MOSFLM |
Data scaling software | MOSFLM |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 43.700 |
High resolution limit [Å] | 1.700 |
Number of reflections | 69651 |
<I/σ(I)> | 21.6 |
Completeness [%] | 100.0 |
Redundancy | 15.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 1.2 M NaH2PO4/0.8 M K2HPO4, 0.1 M CAPS pH 10.5, 0.2 M Li2SO4, 0.1 M ZnCl2 |