4YJ1

Crystal structure of T. brucei MRB1590-ADP bound to poly-U RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	I 4 2 2
Unit cell lengths	215.433, 215.433, 100.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.800 - 2.050
R-factor	0.1748
Rwork	0.173
R-free	0.20290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.905
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	41.800
High resolution limit [Å]	2.050
Number of reflections	70357
<I/σ(I)>	12
Completeness [%]	95.6
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3350, magnesium chloride