4YIJ
Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-01-16 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.960, 54.283, 108.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.270 - 1.640 |
| R-factor | 0.1583 |
| Rwork | 0.157 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.807 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.660 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.027 | 0.223 |
| Total number of observations | 546969 | |
| Number of reflections | 34804 | |
| <I/σ(I)> | 30.12 | 5.34 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 15.09 | 6.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 25% MPD, 25mM potassium phosphate, calcium chloride, pdTp |
