4YIF
Crystal structure of Rv0880
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-01-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.970, 69.600, 70.320 |
| Unit cell angles | 103.71, 111.06, 105.83 |
Refinement procedure
| Resolution | 37.108 - 2.002 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hr3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.222 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.071 | 0.543 |
| Number of reflections | 55124 | |
| <I/σ(I)> | 12.3 | 2.4 |
| Completeness [%] | 94.3 | 94.3 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 289 | 0.1M HEPES, 5%(V/V) Hexylene Glycol ,12%(W/V) PEG6000 |
