4YI8
Crystal structure of non-myristoylated E153A recoverin at 1.2 A resolution with calcium ions bound to EF-hands 2 and 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 |
Unit cell lengths | 84.160, 84.160, 59.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.000 - 1.200 |
R-factor | 0.1218 |
Rwork | 0.121 |
R-free | 0.14010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mlw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.307 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.260 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.068 | 0.346 |
Number of reflections | 64447 | |
<I/σ(I)> | 17.3 | 3.9 |
Completeness [%] | 99.9 | 99.1 |
Redundancy | 7.3 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 296 | 2.5 M Ammonium sulphate |