4YI7

Anthranilate bound at active site of anthranilate phosphoribosyl transferase from Acinetobacter (AnPRT; TrpD)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2014-12-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	C 2 2 21
Unit cell lengths	60.997, 70.830, 169.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.289 - 1.853
R-factor	0.2247
Rwork	0.223
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gtn
RMSD bond length	0.007
RMSD bond angle	0.936
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER ((phaser_mr: 2.5.6))
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.910
High resolution limit [Å]	1.850	1.850
Rmerge	0.025	0.402
Number of reflections	31276
<I/σ(I)>	28.7	2.7
Completeness [%]	99.9	99
Redundancy	7.2	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.96	291.15	PEG 8000, sodium cacodylate, magnesium acetate