4YI0
Structure of mouse importin a1 bound to Pom121NLS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-19 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.337, 89.753, 97.868 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.072 - 1.806 |
R-factor | 0.1827 |
Rwork | 0.182 |
R-free | 0.20990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q5u |
RMSD bond length | 0.007 |
RMSD bond angle | 1.094 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1696) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.072 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.501 | |
Number of reflections | 60036 | |
<I/σ(I)> | 34.7 | 3.2 |
Completeness [%] | 97.8 | 98.3 |
Redundancy | 3.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate tribasic dihydrate, 0.7 M sodium citrate tribasic dihydrate, 25 mM DTT |