4YHR

Crystal Structure of Yeast Proliferating Cell Nuclear Antigen

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-21
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 3
Unit cell lengths	124.259, 124.259, 124.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.465 - 2.950
R-factor	0.2477
Rwork	0.246
R-free	0.27330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1plq
RMSD bond length	0.004
RMSD bond angle	0.710
Data scaling software	d*TREK (9.9.9.5L)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.470	37.470	3.060
High resolution limit [Å]	2.950	6.350	2.950
Rmerge	0.109	0.070	0.893
Total number of observations	302153	29976	30287
Number of reflections	13746
<I/σ(I)>	12.2	32.8	2.1
Completeness [%]	100.0	100	100
Redundancy	21.82	20.37	22.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Protein solution: 15 mg/mL protein in 20 mM HEPES and 150 mM NaCL at pH 7.5. Crystallization solution: 2.2M ammonium sulfate, 0.2M ammonium fluoride. Hanging drop was 50% protein solution, 50% crystallization solution