4YHD
Staphylococcal alpha-hemolysin H35A mutant monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.930, 128.910, 135.280 |
| Unit cell angles | 90.00, 91.57, 90.00 |
Refinement procedure
| Resolution | 37.393 - 2.801 |
| R-factor | 0.2174 |
| Rwork | 0.215 |
| R-free | 0.26270 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.777 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.130 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 61440 |
| <I/σ(I)> | 10 |
| Completeness [%] | 95.8 |
| Redundancy | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | sodium cacodylate. calcium chloride, supermine, 2-propanol, ethylene glycol |
