4YHB
Crystal structure of a siderophore utilization protein from T. fusca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.541, 75.067, 73.904 |
| Unit cell angles | 90.00, 102.62, 90.00 |
Refinement procedure
| Resolution | 36.744 - 1.889 |
| R-factor | 0.1903 |
| Rwork | 0.189 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gpj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.303 |
| Data reduction software | XDS (10.5.8) |
| Data scaling software | Aimless (0.5.4) |
| Phasing software | PHASER (2.1.2) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.530 | 1.957 |
| High resolution limit [Å] | 1.889 | 1.889 |
| Rmerge | 0.057 | 0.440 |
| Number of reflections | 54927 | |
| <I/σ(I)> | 13.3 | 2.64 |
| Completeness [%] | 99.3 | 94.18 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 0.95 M ammonia sulfate, 0.1 M Tris-HCl pH 6.6 and 35% v/v 2-methyl-2, 4-pentanediol |
