4YH8

Structure of yeast U2AF complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-11
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	91.616, 112.704, 57.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.545 - 1.700
R-factor	0.1924
Rwork	0.191
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.053
Data scaling software	HKL-2000
Phasing software	PHENIX ((phenix.refine: 1.9_1692))
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	32806
<I/σ(I)>	33.8	1.5
Completeness [%]	99.1	90.4
Redundancy	8.4	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG 1000, 0.1M MES