4YH2

Glutathione Transferase E6 from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	176.350, 58.860, 122.810
Unit cell angles	90.00, 128.25, 90.00

Refinement procedure

Resolution	96.440 - 1.720
R-factor	0.1587
Rwork	0.157
R-free	0.18840
RMSD bond length	0.006
RMSD bond angle	1.057
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	xia2
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	96.440
High resolution limit [Å]	1.720
Number of reflections	105003
<I/σ(I)>	12.3
Completeness [%]	99.8
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		288	PEG3350, Sodium thiocyanate