4YGT

Crystal structure of a DUF4309 family protein (YjgB) from Bacillus subtilis subsp. subtilis str. 168 at 2.13 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793,0.7514,0.9791
Spacegroup name	P 61
Unit cell lengths	90.830, 90.830, 41.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.416 - 2.130
R-factor	0.1933
Rwork	0.191
R-free	0.23130
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.150
Data scaling software	XSCALE (January 10, 2014 BUILT=20140307)
Phasing software	SHELX
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.416	45.416	2.210
High resolution limit [Å]	2.130	4.570	2.130
Rmerge	0.124	0.045	1.635
Rmeas	0.130	0.048	1.726
Total number of observations	120232
Number of reflections	11231	1181	1159
<I/σ(I)>	14.58	46.5	1.9
Completeness [%]	99.9	99.5	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M sodium sulfate, 20% polyethylene glycol 3350