4YGM
Vaccinia virus his-D4/A20(1-50) in complex with uracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.786, 92.786, 146.744 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.360 - 1.850 |
| R-factor | 0.19229 |
| Rwork | 0.191 |
| R-free | 0.22248 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4od8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.628 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.000 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.052 | 0.597 |
| Number of reflections | 63182 | |
| <I/σ(I)> | 23.2 | 3.6 |
| Completeness [%] | 96.4 | 94 |
| Redundancy | 7.5 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 0.1M bicine, 1.5M ammonium sulfate |
