4YGC
Crystal structure of ERGIC-53/MCFD2, monoclinic calcium-bound form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-07-24 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.616, 168.892, 73.955 |
| Unit cell angles | 90.00, 119.86, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a4u |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.509 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.056 | 0.188 |
| Number of reflections | 61313 | |
| <I/σ(I)> | 38.6 | 9.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 17% PEG8000, 8% ethylene glycol, 0.1 M Tris-HCl |
