Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9770 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 165.750, 165.750, 263.846 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.070 - 2.750 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.23000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 13.595 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 56095 | |
| <I/σ(I)> | 18.8 | 1.7 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 7.3 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 299 | 0.35 M POTASSIUM CHLORIDE, 3 MM DITHIOTHREITOL, 10 MM HEPES PH 7.4, 150 MM NACL, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 299K |
