4YG4
HipB-O1-O1* complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.650, 69.800, 78.300 |
| Unit cell angles | 90.00, 93.10, 90.00 |
Refinement procedure
| Resolution | 78.300 - 3.500 |
| Rwork | 0.267 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dnv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.359 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.300 | |
| High resolution limit [Å] | 3.500 | |
| Rmerge | 0.723 | |
| Number of reflections | 6721 | |
| <I/σ(I)> | 7.5 | 1.5 |
| Completeness [%] | 96.0 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 27% PEG8000, 0.1 M MES, pH 6.5 |
