4YFY
X-ray structure of the Viof N-formyltransferase from Providencia alcalifaciens O30 in complex with THF and TDP-Qui4N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-23 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 132.000, 132.000, 161.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.18948 |
| Rwork | 0.187 |
| R-free | 0.24391 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yfv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.121 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.237 |
| Number of reflections | 42792 | |
| <I/σ(I)> | 14.3 | 2.4 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 5.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 8-10% PEG-8000, 1 M tetramethylammonium chloride 100 mM Hepes |
