4YFW

Structural basis of glycan recognition in neonate-specific rotaviruses

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977408
Spacegroup name	P 1 21 1
Unit cell lengths	36.530, 71.940, 58.050
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	36.530 - 1.660
R-factor	0.2217
Rwork	0.220
R-free	0.25480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4drv
RMSD bond length	0.008
RMSD bond angle	1.130
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.530	1.750
High resolution limit [Å]	1.660	1.660
Rmerge	0.080	0.278
Number of reflections	34723
<I/σ(I)>	11.3	4.3
Completeness [%]	98.1	97.2
Redundancy	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	20% PEG 8000 0.1 M CHES