4YFS

Structure of the synthetic Duffy Binding Protein (DBP) antigen DEKnull relevant for malaria vaccine design

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2014-09-18
Detector	RDI CMOS_8M
Wavelength(s)	1.000087
Spacegroup name	P 1 21 1
Unit cell lengths	55.630, 37.350, 78.200
Unit cell angles	90.00, 108.77, 90.00

Refinement procedure

Resolution	26.536 - 2.100
R-factor	0.222
Rwork	0.218
R-free	0.25880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rrc
RMSD bond length	0.004
RMSD bond angle	0.749
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.537	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.116	0.460
Number of reflections	17538
<I/σ(I)>	8.51	2.11
Completeness [%]	96.7	97.5
Redundancy	2.93	2.74

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M di-sodium tartrate, 20% PEG 3350, and microseeded into 0.2 M lithium chloride, 20% PEG 3350