4YFO
beta1_ex1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-26 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.180, 105.180, 323.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.680 - 3.390 |
| R-factor | 0.19733 |
| Rwork | 0.196 |
| R-free | 0.23339 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.522 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.830 | 3.480 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmerge | 0.129 | 0.585 |
| Number of reflections | 9607 | |
| <I/σ(I)> | 14.3 | 4.3 |
| Completeness [%] | 96.8 | 98.9 |
| Redundancy | 9.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 90 mM Bis Tris pH 5.5, 2.7 M sodium chloride |
