4YFH
HU38-20bp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 93.15 |
Detector technology | CCD |
Collection date | 2013-01-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 75.010, 75.010, 221.980 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.960 - 3.490 |
R-factor | 0.2223 |
Rwork | 0.220 |
R-free | 0.26530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mul |
RMSD bond length | 0.010 |
RMSD bond angle | 1.470 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 110.990 | 3.497 |
High resolution limit [Å] | 3.485 | 3.485 |
Rmerge | 0.298 | 1.924 |
Total number of observations | 104372 | |
Number of reflections | 5242 | |
<I/σ(I)> | 13.4 | 2.257 |
Completeness [%] | 99.9 | 100 |
Redundancy | 19.9 | 21.76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M Bis-Tris pH 5.8, 30% PEG MME 550, 0.05 M CaCl2 |