4YFE

Crystal structure of PTP delta Fn1-Fn2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-17
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	51.909, 65.081, 133.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.404 - 1.972
R-factor	0.2048
Rwork	0.204
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dju 2dlh
RMSD bond length	0.006
RMSD bond angle	1.321
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.970	1.970
Rmerge		0.331
Number of reflections	32731
<I/σ(I)>	26.6	4.4
Completeness [%]	99.8	99.7
Redundancy	10.7	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	20% PEG 3350, 0.1M MgCl2, 0.1M BisTris