4YF5
Crystal structure of Rv1284 in the presence of polycarpine at acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-15 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95375 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 103.289, 153.932, 159.098 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.561 - 2.000 |
| R-factor | 0.2004 |
| Rwork | 0.197 |
| R-free | 0.25950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ylk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.113 |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.600 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.459 | |
| Number of reflections | 81481 | |
| <I/σ(I)> | 7.3 | 1.6 |
| Completeness [%] | 99.2 | 94.9 |
| Redundancy | 13.7 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 15% PEG 400, 0.1 M NaH2PO4/Na-citrate, 10 mM polycarpine |
