Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 |
| Unit cell lengths | 136.592, 136.592, 33.315 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.150 |
| R-factor | 0.18978 |
| Rwork | 0.188 |
| R-free | 0.21747 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lsg |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.707 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.110 | 0.918 |
| Number of reflections | 19790 | |
| <I/σ(I)> | 17 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 100 mM Bis-Tris, 20% w/v PEG5000 MME, 20% ethanol |
