4YF0
HU38-19bp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 93.15 |
| Detector technology | CCD |
| Collection date | 2013-01-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 74.890, 74.890, 220.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.860 - 2.790 |
| R-factor | 0.2481 |
| Rwork | 0.247 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mul |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.103 | 2.800 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.106 | 1.913 |
| Total number of observations | 386391 | |
| Number of reflections | 9847 | |
| <I/σ(I)> | 35.1 | 2.199 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 39.2 | 31.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M Bis-Tris pH 5.8, 30% PEG MME 550, 0.05 M CaCl2 |
