4YEW
HUab-19bp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2013-01-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 84.460, 84.460, 63.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.720 - 2.683 |
| R-factor | 0.2515 |
| Rwork | 0.250 |
| R-free | 0.29030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mul |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.461 | 2.692 |
| High resolution limit [Å] | 2.683 | 2.683 |
| Rmerge | 0.050 | 0.820 |
| Total number of observations | 56788 | |
| Number of reflections | 6882 | |
| <I/σ(I)> | 28.4 | 2.58 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.3 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.1M Bis-Tris pH 6.5, 30% PEG MME 550, 0.05 M CaCl2 |
