4YEQ
L4b Domain of Human Laminin alpha-2
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979588, 0.979804, 0.977735 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 161.107, 161.107, 78.929 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.899 - 3.200 |
R-factor | 0.2162 |
Rwork | 0.212 |
R-free | 0.27860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yep |
RMSD bond length | 0.012 |
RMSD bond angle | 1.367 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1688)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.900 | 3.314 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 10031 | |
<I/σ(I)> | 15.9 | 1.8 |
Completeness [%] | 96.6 | 94.8 |
Redundancy | 9.6 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM sodium malonate, 100 mM MgCl2, 10% PEG 200, 100 mM Tris, pH 7.0 |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM sodium malonate, 100 mM MgCl2, 10% PEG 200, 100 mM Tris, pH 7.0 |