4YEB
Structural characterization of a synaptic adhesion complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-30 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.934, 121.934, 83.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.430 - 3.190 |
| R-factor | 0.2651 |
| Rwork | 0.257 |
| R-free | 0.33570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4RMK and 4V2E |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.330 | 3.410 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.055 | 0.474 |
| Number of reflections | 12230 | |
| <I/σ(I)> | 9.4 | 1.5 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 6 % PEG 3350, 0.2 M NaNO3 |
