4YEA
Crystal structure of cisplatin bound to a human copper chaperone (dimer) - new refinement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2009-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 65 |
| Unit cell lengths | 78.289, 78.289, 54.335 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.630 - 2.140 |
| R-factor | 0.1621 |
| Rwork | 0.160 |
| R-free | 0.19740 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3iwx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.469 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.078 | 0.401 |
| Number of reflections | 10562 | |
| <I/σ(I)> | 23.4 | 3.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 1.5 M LITHIUM SULFATE, 0.1M MES, 50 MM NACL, PH 6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 294K |
