4YE9
The crystal structure of the G45V mutant of human GlnRS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03318 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 336.940, 58.525, 86.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.498 - 2.700 |
| R-factor | 0.2293 |
| Rwork | 0.228 |
| R-free | 0.26230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ye6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.603 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.116 | 0.059 | |
| Rmeas | 0.126 | 0.068 | |
| Rpim | 0.099 | 0.033 | 0.975 |
| Total number of observations | 223431 | ||
| Number of reflections | 59517 | ||
| <I/σ(I)> | 4.6 | 0.73 | |
| Completeness [%] | 98.7 | 96.7 | 98.4 |
| Redundancy | 3.8 | 4 | 3.3 |
| CC(1/2) | 0.994 | 0.008 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | ammonium acetate, bis-tris, PEG 20,000, EDTA |
