4YE8
The crystal structure of the Y57H mutant of human GlnRS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 333.088, 58.259, 85.132 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.874 - 3.300 |
| R-factor | 0.2254 |
| Rwork | 0.221 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ye6 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.927 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
| Rmerge | 0.189 | 0.031 | |
| Rmeas | 0.197 | 0.036 | |
| Rpim | 0.118 | 0.017 | 0.714 |
| Total number of observations | 124797 | ||
| Number of reflections | 25952 | ||
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 99.8 | 96.9 | 100 |
| Redundancy | 4.8 | 4.1 | 4.9 |
| CC(1/2) | 0.998 | 0.328 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | ammonium acetate, bis-tris, 17% (w/v) PEG 10,000 |
