4YE5
The crystal structure of a peptidoglycan synthetase from Bifidobacterium adolescentis ATCC 15703
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.956, 85.709, 227.476 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.387 - 2.052 |
R-factor | 0.1908 |
Rwork | 0.189 |
R-free | 0.22430 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.033 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.106 | 0.765 |
Number of reflections | 102844 | |
<I/σ(I)> | 36.37 | 2.58 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1M Bis-Tris Propane:NaOH, 1.5M Ammonium Sulfate |