4YE4
Crystal Structure of Neutralizing Antibody HJ16 in Complex with HIV-1 gp120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2013-04-14 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 87.712, 99.037, 180.426 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.900 - 2.720 |
R-factor | 0.1896 |
Rwork | 0.187 |
R-free | 0.24180 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.900 | |
High resolution limit [Å] | 2.720 | 2.720 |
Number of reflections | 20510 | |
<I/σ(I)> | 1.98 | |
Completeness [%] | 94.4 | |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M imidazole pH 6.5 10 % (W/V) PEG 8000 5% isopropanol |