4YE0
Stress-induced protein 1 truncation mutant (43 - 140) from Caenorhabditis elegans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.390, 50.820, 130.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.950 - 2.100 |
R-factor | 0.23171 |
Rwork | 0.230 |
R-free | 0.25948 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.060 | 0.527 |
Number of reflections | 14754 | |
<I/σ(I)> | 16.3 | 2.9 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 33 mM CAPS, 17 mM BisTRIS, 17 mM ammoniumsulfate, 10% pentaerythritol ethoxylate |