4YDW
CRYSTAL STRUCTURE OF ANTI-IL4 DARPIN 44C12V5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-11 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.240, 60.740, 81.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.744 - 1.900 |
| R-factor | 0.17206 |
| Rwork | 0.170 |
| R-free | 0.21229 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ydy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.052 | 0.294 |
| Number of reflections | 22846 | |
| <I/σ(I)> | 43.5 | 7.8 |
| Completeness [%] | 94.2 | 71.5 |
| Redundancy | 14.1 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 293 | 23% PEG 3350, 0.2 M DI-AMMONIUM CITRATE, 3% DIOXANE, 3% ISOPROPANOL |
