4YDV
STRUCTURE OF THE ANTIBODY 7B2 THAT CAPTURES HIV-1 VIRIONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-10-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.434, 76.281, 127.627 |
| Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
| Resolution | 30.800 - 2.700 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.992 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.800 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.037 | 0.676 |
| Number of reflections | 39428 | |
| <I/σ(I)> | 31 | 1.6 |
| Completeness [%] | 99.1 | 98.7 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | 6% PEG 20,000, 5% 2-PROPANOL, 0.1 M CITRIC ACID PH 3.5 |
