4YDT
Beta1 synthetic solenoid protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.04030 |
Spacegroup name | H 3 2 |
Unit cell lengths | 102.370, 102.370, 290.430 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.670 - 3.310 |
R-factor | 0.2571 |
Rwork | 0.255 |
R-free | 0.30299 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.564 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.670 | 3.400 |
High resolution limit [Å] | 3.310 | 3.310 |
Rmerge | 0.160 | 0.950 |
Number of reflections | 8605 | |
<I/σ(I)> | 5.2363 | 1.08 |
Completeness [%] | 94.8 | 96.87 |
Redundancy | 7.71 | 7.92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 160 mM magnesium chloride, 80 mM sodium cacodylate pH 6.5, 40% v/v PEG 200 |