4YD8
Bardet-Biedl Syndrome 9 Protein (aa1-407), Homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 136.417, 81.878, 85.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.936 - 1.800 |
| R-factor | 0.1697 |
| Rwork | 0.169 |
| R-free | 0.19970 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.119 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1951) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.990 | |
| Number of reflections | 171175 | |
| <I/σ(I)> | 23.3 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 291 | 10% w/v PEG 4000, 0.1M tri-sodium citrate pH 6.1, and 0.15M ammonium sulfate |
