4YCZ

Y-COMPLEX HUB (NUP85-NUP120-NUP145C-SEC13 COMPLEX) FROM M. THERMOPHILA (A.K.A. T. HETEROTHALLICA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-22
Detector	PSI PILATUS 6M
Wavelength(s)	0.97890
Spacegroup name	C 1 2 1
Unit cell lengths	104.984, 212.022, 170.643
Unit cell angles	90.00, 107.21, 90.00

Refinement procedure

Resolution	163.000 - 4.100
R-factor	0.3218
Rwork	0.319
R-free	0.35830
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.640
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_1951))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	163.000	4.250
High resolution limit [Å]	4.100	4.100
Rmerge		1.000
Number of reflections	53692
<I/σ(I)>	8.9	0.9
Completeness [%]	98.2	93.5
Redundancy	6.1	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.23	291	50mM Tris-HCL pH 8.23, 0.7M Ammonium Sulfate, 20mM EDTA