4YCQ
Beta1 synthetic solenoid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.04030 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 125.420, 125.420, 47.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.053 - 2.405 |
| R-factor | 0.1726 |
| Rwork | 0.171 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yc5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.963 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.920 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.910 |
| Number of reflections | 16977 | |
| <I/σ(I)> | 5.7859 | 3.01 |
| Completeness [%] | 99.2 | 99.02 |
| Redundancy | 10.66 | 11.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 291 | 0.1 M CAPS pH 10.5, 40% (v/v) MPD |
