4YCP
E. coli dihydrouridine synthase C (DusC) in complex with tRNATrp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.96861 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.516, 98.516, 231.172 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.260 - 2.550 |
| R-factor | 0.2095 |
| Rwork | 0.208 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bfa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.260 | 2.660 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.141 | 1.099 |
| Number of reflections | 22329 | |
| <I/σ(I)> | 8.8 | 1.5 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Bis-Tris pH 6.5, 0.2 M Li2SO4 26% PEG 2K MME |
