4YC1

Structure of the human TSG101-UEV domain in the P321 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97779
Spacegroup name	P 3 2 1
Unit cell lengths	169.739, 169.739, 39.707
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.670 - 2.000
R-factor	0.1655
Rwork	0.164
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f0r
RMSD bond length	0.013
RMSD bond angle	1.376
Data reduction software	XDS
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.670	19.670	2.060
High resolution limit [Å]	2.000	8.490	2.000
Rmerge	0.059	0.030	0.559
Total number of observations	381505	4499	29916
Number of reflections	44232
<I/σ(I)>	22.6	58.5	4.5
Completeness [%]	99.7	91.5	100
Redundancy	8.6	7.9	8.3
CC(1/2)	0.999	0.999	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	25% PEG4k, 0.2 Ammonium sulphate, 0.1 M Sodium Acetate