4YBG

Crystal structure of the MAEL domain of Drosophila melanogaster Maelstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-27
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	71.892, 71.892, 88.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.353 - 1.602
R-factor	0.1955
Rwork	0.194
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	The MAEL domain solved by Zn-SAD
RMSD bond length	0.011
RMSD bond angle	1.289
Data reduction software	XDS
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.600	1.600
Number of reflections	31077
<I/σ(I)>	18.7
Completeness [%]	99.8
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, ammonium acetate