4YBB

High-resolution structure of the Escherichia coli ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	212.170, 433.890, 624.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.388 - 2.100
R-factor	0.2186
Rwork	0.218
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v9d
RMSD bond length	0.008
RMSD bond angle	1.270
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	69.388		2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.154	0.046	3.386
Rmeas	0.169	0.050	4.105
Total number of observations	13135436
Number of reflections	3309372	43452	107902
<I/σ(I)>	4.77	22.01	0.16
Completeness [%]	86.9	97.8	38.4
Redundancy	3.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD