4YBB
High-resolution structure of the Escherichia coli ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 212.170, 433.890, 624.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.388 - 2.100 |
R-factor | 0.2186 |
Rwork | 0.218 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v9d |
RMSD bond length | 0.008 |
RMSD bond angle | 1.270 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 69.388 | 2.050 | |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.154 | 0.046 | 3.386 |
Rmeas | 0.169 | 0.050 | 4.105 |
Total number of observations | 13135436 | ||
Number of reflections | 3309372 | 43452 | 107902 |
<I/σ(I)> | 4.77 | 22.01 | 0.16 |
Completeness [%] | 86.9 | 97.8 | 38.4 |
Redundancy | 3.97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD |