4YBA
The structure of the C.Kpn2I controller protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2014-01-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.890, 44.730, 109.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.370 - 1.700 |
R-factor | 0.19209 |
Rwork | 0.190 |
R-free | 0.22604 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ir6 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.562 |
Data reduction software | MOSFLM (7.0.9) |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.460 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.078 | 0.611 |
Number of reflections | 17568 | |
<I/σ(I)> | 7.8 | 2.3 |
Completeness [%] | 86.7 | 89 |
Redundancy | 3.4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2 LiSO4, 0.1 M Bis Tris methane pH5.5, 25% PEG3350; cryoprotection: 30% PEG3350, 10% glycerol |