4YB6
Adenosine triphosphate phosphoribosyltransferase from Campylobacter jejuni in complex with the inhibitors AMP and histidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-04-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.959 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.871, 124.906, 92.809 |
| Unit cell angles | 90.00, 115.86, 90.00 |
Refinement procedure
| Resolution | 39.580 - 1.980 |
| R-factor | 0.19621 |
| Rwork | 0.195 |
| R-free | 0.21477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yb5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.580 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.059 | 0.422 |
| Number of reflections | 127994 | |
| <I/σ(I)> | 2 | 3.1 |
| Completeness [%] | 98.1 | 96.9 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | Tris, Magnesium Chloride, PEG4000, AMP, histidine |
