4YB1

20A Mutant c-di-GMP Vc2 Riboswitch bound with 3',3'-cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	51.738, 46.836, 80.791
Unit cell angles	90.00, 90.44, 90.00

Refinement procedure

Resolution	43.722 - 2.081
R-factor	0.2223
Rwork	0.218
R-free	0.29850
RMSD bond length	0.012
RMSD bond angle	1.223
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	2.140
High resolution limit [Å]	2.080	2.080
Rmerge	0.059
Number of reflections	17467
<I/σ(I)>	11.3	0.6
Completeness [%]	75.2	42.2
Redundancy	2	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M Na-citrate pH 5.5, 5% PEG1000, 35% iso-propanol