4YB0
3',3'-cGAMP riboswitch bound with c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.829, 44.901, 68.210 |
| Unit cell angles | 90.00, 103.33, 90.00 |
Refinement procedure
| Resolution | 85.100 - 2.121 |
| R-factor | 0.2297 |
| Rwork | 0.228 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yaz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.457 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.100 | 2.240 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.044 | |
| Number of reflections | 28937 | |
| <I/σ(I)> | 16.3 | 1.3 |
| Completeness [%] | 98.1 | 97.9 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Na/K-phosphate, pH 6.2-6.6, 0.2 M NaCl and 40-45% PEG400 |
