4YAU

Reduced CYPOR mutant - G141del/E142N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-04-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	101.557, 115.901, 118.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.680 - 2.200
R-factor	0.201
Rwork	0.201
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1amo
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.680
High resolution limit [Å]	2.200
Number of reflections	68312

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	PEG 4000, Sodium Acetate, HEPES